Singlet glycine riboswitches bind ligand as well as tandem riboswitches
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چکیده
منابع مشابه
Engineering and characterization of fluorogenic glycine riboswitches
A set of 12 fluorogenic glycine riboswitches with different thermodynamic and kinetic response properties was engineered. For the design of functional riboswitches, a three-part RNA approach was applied based on the idea of linking a RNA sensor, transmitter and actuator part together. For the RNA sensor and actuator part, we used the tandem glycine aptamer structure from Bacillus subtillis, and...
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Guanine riboswitches negatively modulate transcription upon guanine binding. The aptamer domain is organized around a three-way junction which forms the ligand binding site. Using currently available 89 guanine aptamer sequences, a consensus secondary structure is deduced and reveals differences from the previously identified aptamer consensus. Three positions are found to display different nuc...
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RNA aptamers readily recognize small organic molecules, polypeptides, as well as other nucleic acids in a highly specific manner. Many such aptamers have evolved as parts of regulatory systems in nature. Experimental selection techniques such as SELEX have been very successful in finding artificial aptamers for a wide variety of natural and synthetic ligands. Changes in structure and/or stabili...
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Riboswitches are common cis-acting regulatory elements in bacteria. They are made of nascent RNA that changes its conformation in response to direct binding of cognate metabolites. The publication of five high-resolution crystal structures provides a comprehensive view of how riboswitches sense their ligands and points to new challenges in this emerging field.
متن کاملBacterial riboswitches
This wikipedia entry provides an extensive description of natural and engineered riboswitches. Most known riboswitches have been found in bacteria. This webpage provides a link to an electronic thesis file describing a riboswitch that responds to the herbicide atrazine. This web article discusses synthetic riboswitches. These are tools that researchers can use to control bacterial metabolism. T...
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ژورنال
عنوان ژورنال: RNA
سال: 2016
ISSN: 1355-8382,1469-9001
DOI: 10.1261/rna.057935.116